Syllabus
|
Course Code: BP811ET Course Name: Advanced Instrumentation Techniques |
||
| MODULE NO / UNIT | COURSE SYLLABUS CONTENTS OF MODULE | NOTES |
|---|---|---|
| 1 | Nuclear Magnetic Resonance spectroscopy Principles of H-NMR and C-NMR, chemical shift, factors affecting chemical shift, coupling constant, Spin - spin coupling, relaxation, instrumentation and applications Mass Spectrometry- Principles, Fragmentation, Ionization techniques – Electron impact, chemical ionization, MALDI, FAB, Analyzers-Time of flight and Quadrupole, instrumentation, applications |
|
| 2 | Thermal Methods of Analysis: Principles, instrumentation and applications of ThermogravimetricAnalysis (TGA), Differential Thermal Analysis (DTA), Differential Scanning Calorimetry (DSC) X- Ray Diffraction Methods: Origin of X-rays, basic aspects of crystals, X- ray Crystallography, rotating crystal technique, single crystal diffraction,powder diffraction, structural elucidation and applications. |
|
| 3 | Calibration and validation-as per ICH and USFDA guidelines Calibration of following Instruments Electronic balance, UV-Visible spectrophotometer, IR spectrophotometer, Fluorimeter, Flame Photometer, HPLC and GC |
|
| 4 | Radio immune assay: Importance, various components, Principle, different methods, Limitation and Applications of Radio immuno assay Extraction techniques: General principle and procedure involved in the solid phase extraction and liquid-liquid extraction | |
| 5 | Hyphenated techniques-LC-MS/MS, GC-MS/MS, HPTLC-MS. | |
