Syllabus
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Course Code: BP701T Course Name: Instrumental Methods of Analysis – Theory |
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| MODULE NO / UNIT | COURSE SYLLABUS CONTENTS OF MODULE | NOTES |
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| 1 | UV Visible spectroscopy Electronic transitions, chromophores, auxochromes, spectral shifts, solvent effect on absorption spectra, Beer and Lambert’s law, Derivation and deviations. Instrumentation - Sources of radiation, wavelength selectors, sample cells, detectors- Photo tube, Photomultiplier tube, Photo voltaic cell, Silicon Photodiode. Applications - Spectrophotometric titrations, Single component and multi component analysis Fluorimetry Theory, Concepts of singlet, doublet and triplet electronic states, internal and external conversions, factors affecting fluorescence, quenching, instrumentation and applications |
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| 2 | IR spectroscopy Introduction, fundamental modes of vibrations in poly atomic molecules, sample handling, factors affecting vibrations Instrumentation - Sources of radiation, wavelength selectors, detectors - Golay cell, Bolometer, Thermocouple, Thermister, Pyroelectric detector and applications Flame Photometry-Principle, interferences, instrumentation and applications Atomic absorption spectroscopy- Principle, interferences, instrumentation and applications Nepheloturbidometry- Principle, instrumentation and applications |
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| 3 | Introduction to chromatography Adsorption and partition column chromatography-Methodology, advantages, disadvantages and applications. Thin layer chromatography- Introduction, Principle, Methodology, Rf values, advantages, disadvantages and applications. Paper chromatography-Introduction, methodology, development techniques, advantages, disadvantages and applications Electrophoresis– Introduction, factors affecting electrophoretic mobility, Techniques of paper, gel, capillary electrophoresis, applications |
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| 4 | Gas chromatography - Introduction, theory, instrumentation, derivatization, temperature programming, advantages, disadvantages and applications High performance liquid chromatography (HPLC)-Introduction, theory, instrumentation, advantages and applications. |
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| 5 | Ion exchange chromatography- Introduction, classification, ion exchange resins, properties, mechanism of ion exchange process, factors affecting ion exchange, methodology and applications Gel chromatography- Introduction, theory, instrumentation and applications Affinity chromatography- Introduction, theory, instrumentation and applications |
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