Syllabus

Course Code: BP307P    Course Name: Pharmaceutical Microbiology – Practical

MODULE NO / UNIT COURSE SYLLABUS CONTENTS OF MODULE NOTES
1 1. Introduction and study of different equipments and processing, e.g., B.O.D. incubator, laminar flow, aseptic hood, autoclave, hot air sterilizer, deep freezer, refrigerator, microscopes used in experimental microbiology.
2 2. Sterilization of glassware, preparation and sterilization of media.
3 3. Sub culturing of bacteria and fungus. Nutrient stabs and slants preparations.
4 4. Staining methods- Simple, Grams staining and acid fast staining (Demonstration with practical).
5 5. Isolation of pure culture of micro-organisms by multiple streak plate technique and other techniques.
6 6. Microbiological assay of antibiotics by cup plate method and other methods
7 7. Motility determination by Hanging drop method.
8 8. Sterility testing of pharmaceuticals.
9 9. Bacteriological analysis of water
10 10. Biochemical test.
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