Syllabus
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Course Code: MTBT-117 Course Name: Bio-analytical Techniques Lab |
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| MODULE NO / UNIT | COURSE SYLLABUS CONTENTS OF MODULE | NOTES |
|---|---|---|
| 1 | Concept of pH, preparation of buffers, measurement of pH. | |
| 2 | Centrifugation: Principle and technique. | |
| 3 | Chromatographic techniques: TLC, Gel Filtration Chromatography, Ion exchange Chromatography, Affinity Chromatography. | |
| 4 | Electrophoretic techniques - Agarose and PAGE (nucleic acids and proteins). | |
| 5 | Immunochemical techniques – general principles and applications of immunodiffusion, immunoelectrophoresis, radioimmunoassay, enzyme linked immunosorbent assay, fluorescence immunoassay. | |
| 6 | Spectroscopy Concepts of spectroscopy, Visible and UV spectroscopy, Laws of photometry. Beer-Lamberts law, Principles and applications of colorimetry. | |
| 7 | Biosensors and their applications. | |
