Syllabus
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Course Code: *Program Elective -II MTBT-115 Course Name: Protein Engineering |
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| MODULE NO / UNIT | COURSE SYLLABUS CONTENTS OF MODULE | NOTES |
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| 1 | Protein Structure Characterization: Introduction to protein engineering, structure and properties of amino acids, primary, secondary, tertiary and quaternary structure of proteins, analysis of protein structure by CD spectroscopy, NMR, X ray diffraction crystallography, | |
| 2 | Protein Structure Prediction: Protein prediction of protein structure using bioinformatics approach, protein sequence and structure
relationship, predicting the conformation of proteins from sequence data Protein Folding – Molecular Energy and Forces, Strategies for design of novel proteins-strategies for the design of structure and function, computer methods in protein modeling, mutations and their effects on protein folding, |
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| 3 | Protein Engineering Strategies and Techniques: protein engineering - methodology, application and interpretation, Directed
evolution and Rational design (Computer modeling). Protein Evolution - Cell surface and phage display technologies, Cell-free protein engineering technologies |
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| 4 | Engineering the Proteins and Their Application: Effect of amino acids on structure of proteins, prediction of structure function
relations of enzymes and other proteins, gene shuffling methods such as RACHITT, ITCHY, SCRATCHY Examples of engineered proteins:, Engineering fluorescent proteins/molecular probes, Engineering multi-functional proteins, Antibody engineering |
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