Syllabus
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Course Code: MTBT-103 Course Name: Advances in Bioprocess Engineering |
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| MODULE NO / UNIT | COURSE SYLLABUS CONTENTS OF MODULE | NOTES |
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| 1 | Introduction to Bioprocess Engineering: Historical development of bioprocessing technology, processing and production of recombinant products. Batch and chemostat cultures; Computer simulations; Fed-batch and mixed cultures; Scale-up principles. Transport phenomenon in bioprocess systems. | |
| 2 | Kinetics of substrate utilization and product formation. Ideal reactors for kinetics measurements. High performing reactors and industrial reactors. Kinetics of balanced growth.. Structured kinetic models. Product formation kinetics. Segregated kinetic models of growth and product formation | |
| 3 | Recovery and purification of fermentation products: Liquid-liquid extraction, cell disruption and isolation of non- secreted products, Lyophilization and Spray drying. Membrane based affinity separations; two-phase affinity partitioning; use of reverse micelles in protein separation; chiral separations; molecular imprinting. | |
| 4 | Fermentation Technology: Case studies on production of lactic acid, glutamic acid, penicillin, microbial lipase and protease,
recombinant insulin. Case studies should deal with strain improvement, medium designs, and process optimization. References 1. Biochemical Engineering fundamentals" by J E Bailey and D F Ollis, 2nd ed, McGraw-Hill . 2. "Principles of fermentation technology" by P F Stanbury and A Whitaker, Pergamon press. 3. "Principles of Cell Energetics" : BIOTOL series, Butterworth - Heinemann. 4. "Bioprocess Technology - Kinetics & Reactors" by A Moser, Springer-Verlag. 5. "Biotechnology" Vol.4 Meanning Modeling and Control Ed. K.Schugerl, VCH (1991). 6 "Biotechnology" Vol.3 Bioprocessing Ed.G. Stephanopoulos, VCH (1991). 7. "Biochemical Engineering and Biotechnology Handbook" by B.Atkinson&F.Mavituna, 2nd Ed. Stockton Press (1991). 7. Specific journals and published references. |
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