Syllabus
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Course Code: MTBT-101 Course Name: Genomics and Proteomics |
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| MODULE NO / UNIT | COURSE SYLLABUS CONTENTS OF MODULE | NOTES |
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| 1 | Introduction: Structural organization of genome in Prokaryotes and Eukaryotes; Organelle DNA-mitochondrial, chloroplast; DNA sequencing principles and translation to large scale projects; Next-Gen sequence technology and applications. Recognition of coding and non-coding sequences and gene annotation; Tools for genome analysis- RFLP, DNA fingerprinting, RAPD, PCR,. DNA chips and their use in transcriptome analysis; Mutants and RNAi in functional genomics. | |
| 2 | Genome sequencing projects: Human, microbes, plants and animals; Accessing and retrieving genome project information from web; Identification and classification using molecular markers-16SrRNAtyping/sequencing, EST and SNP's contigs; allele/gene mining; synteny and comparative genomics. Dart | |
| 3 | Proteomics: Protein analysis (includes measurement of concentration, amino acid composition, N-terminal sequencing);2 Delectrophoresis of proteins; Microscale solution isoelectric focusing; Peptide fingerprinting; Protein-protein interactions, Yeast two hybrid system. SAGE. | |
| 4 | Genomic and Proteomic analysis: Metabolomics for elucidating metabolic pathways, Analysis of microarray data; Protein and peptide microarray-based technology; PCR-directed protein insitu arrays; Structural proteomics. Real Time PCR, Platform technologies for screening. | |
