Syllabus

Course Code: MTBT-118    Course Name: Molecular Techniques Lab

MODULE NO / UNIT COURSE SYLLABUS CONTENTS OF MODULE NOTES
1 Extraction of DNA from clinical samples followed by agarose gel electrophoresis.
2 Extraction of double stranded genomic RNA from viral samples
3 Polyacrylamide gel electrophoresis (PAGE) for detection of segmented genomic RNA.
4 Polymerase chain reaction for detection of pathogens in blood/and other clinical samples.
5 RT-PCR for detection of RNA.
6 Detection of food borne pathogenic organisms from food samples using PCR technology.
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