Syllabus
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Course Code: **Program Elective -IV MTBT-116 Course Name: Metagenomics |
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| MODULE NO / UNIT | COURSE SYLLABUS CONTENTS OF MODULE | NOTES |
|---|---|---|
| 1 | Environmental Metagenomics – Introduction; Pure culture and in consortium ; Cultivable and Non-cultivable microbial analysis; Molecular fingerprinting techniques (RFLP, T-RFLP, ARISA, DGGE, rDNA library, and FISH); Stable isotope probing (SIP); Suppressive subtractive hybridization (SSH); Differential expression analysis (DEA); Microarrays & Metagenome sequencing; Nextgeneration sequencing approaches to metagenomics | |
| 2 | Stable isotope probing and oligonucleotide microarrays: Direct linking of microbial populations to specific biodegradation and biotransformation processes by stable isotope probing of biomarkers- PhyloChip & GeoChip-Detection of xenobiotic-degrading bacteria by using oligonucleotide microarrays. | |
| 3 | Library construction and analysis of metagenomic Libraries:Library Cataloging microbes: phylogenetic tree and construction - Construction of a metagenomic library; Analysis of Metagenomic Libraries; Sequence-based Metagenomics Analysis; Function based Metagenomics Analysis; Phylogenetic analysis and Comparative genomics Softwares & Tools | |
| 4 | Metagenomics case studies: Metagenomic analysis of soil microbial communities; marine microbial communities; Microbial Community in Acid Mine Drainage; Bacteriophage; Archaeal Metagenomics: Bioprospecting Novel Genes and Exploring New Concepts; Metagenomics and Its Applications to the Study of the Human Microbiome; Applications of Metagenomics for Industrial Bioproducts | |
