Syllabus
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Course Code: *Program Elective -III MTBT-110 Course Name: Advanced Industrial Biotechnology |
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| MODULE NO / UNIT | COURSE SYLLABUS CONTENTS OF MODULE | NOTES |
|---|---|---|
| 1 | Microbial diversity and strategies for its recovery. Bioprospecting for novel compounds. Screening of microbial isolates for bioactivity. Cultivation of hyperthermophilic and extremely thermo acidophilic microorganisms. Instrumentation and monitoring of bioreactors. Culture and analysis using gel microdrops. | |
| 2 | Experimental design for improvement in fermentation processes. Software applications in fermentation processes. Methods for biocatalysis. Downstream processing. Introduction to bioprocess simulation. Quality assurance and quality control. Concepts of anaerobic fermentation and contract fermentations. | |
| 3 | Introduction to genetic analysis of Streptomyces and Bacillus spp. using tools of recombinant DNA technology. Applications of rDNA technology in thermophiles. Design and assembly of polycistronic operons in Escherichiacoli. In vivo folding of recombinant proteins in E. coli. Expression of G protein coupled receptors inmicroorganisms. Selection of suitable hosts for E. coli optimized for expression of proteins. Mechanism of mRNA degradation in bacteria and their implication for stabilization of heterologous transcripts. Filamentous fungi in industrial biotechnology. Genetics and genomics of Saccharomyces cerevisiae. | |
| 4 | Methods for optimizing industrial enzymes. Cloning and analysis of genes for the biosynthesis of microbial secondary metabolites. Antibiotic resistance mechanisms of bacterial pathogens. Genetics of bacteriocins produced by Lactic acid bacteria and their use in novel industrial applications. Biomarkers and bioreporters to track microbes and monitor their gene expression. Biofilms. Future perspectives in industrial microbial technology. | |
