Syllabus
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Course Code: *Program Elective -III MTBT-106 Course Name: Metabolic Engineering |
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| MODULE NO / UNIT | COURSE SYLLABUS CONTENTS OF MODULE | NOTES |
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| 1 | Introduction: Identification of metabolic regulation. Basic concepts of Metabolic Engineering – Overview of cellular metabolism – Different models for cellular reactions, induction – Jacob Monod model and its regulation, Feedback regulation. Synthesis of Primary metabolites. Amino acid synthesis pathways and its regulation at enzyme level and whole cell level, Alteration of feedback regulation, Limiting accumulation of end products. | |
| 2 | Biosynthesis of Secondary Metabolites. Regulation of secondary metabolite pathways, precursor effects, prophase, idiophase
relationship, Catabolite regulation by passing control of secondary metabolism, producers and applications of secondary metabolites. Bioconversions: Applications of Bioconversions, Factors affecting bioconversions, Specificity, Yields, Cometabolism, Mixed or sequential bioconversions, Conversion of insoluble substances |
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| 3 | Regulation of Enzyme Production. Strain selection, Genetic improvement of strains, Gene dosage, metabolic pathway
manipulations to improve fermentation, the modification of existing - or the introduction of entirely new metabolic pathways Metabolic flux. Integration of anabolism and catabolism, metabolic flux analysis and its applications, Experimental determination method of flux distribution, |
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| 4 | Metabolic engineering with Bioinformatics. Metabolic pathway modeling, Analysis of metabolic control and the structure metabolic
networks, Applications of Metabolic Engineering. Application in pharmaceuticals, chemical bioprocess, food technology, agriculture, bioremediation and biomass conversion. |
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