Syllabus
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Course Code: B-ZOO-502 (ii) Course Name: Recent techniques in Zoology |
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| MODULE NO / UNIT | COURSE SYLLABUS CONTENTS OF MODULE | NOTES |
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| 1 | 1. Centrifugation: Basic principles, theory and applications of preparative and analytical
centrifugation, Rotor types, sedimentation co-efficient and care of rotors. 2. Chromatography: Theory, principle and application of column, paper, thin layer, ion-exchange affinity chromatography. 3. Spectroscopy: Lambert Beer’s law, theory, principles and instrumentation of single and double beam UV/Visible spectroscopy. 4. Electrophoresis: Theory and application of SDS-PAGE and Agarose gel electrophoresis. |
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| 2 | 5. Radioisotopic techniques:- Basic concepts of radioisotopy, Theory and applications of GeigerMuller tube, solid and liquid scintillation counters, primary and secondary fluors, safety rules for
radioisotopic studies, biological applications. 6. Microscopy- Principles and techniques of Microscopy, Light Microscopy, Phase contrast, Fluorescence, Stereomicroscope and Electron Microscope (SEM and TEM). Principles and uses of pH Meter, Calorimeter, Microtome. 7. PCR-General principle, working and its applications. |
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