Syllabus
|
Course Code: BOT602 Course Name: Research Methodology- II |
||
| MODULE NO / UNIT | COURSE SYLLABUS CONTENTS OF MODULE | NOTES |
|---|---|---|
| 1 | Overview of Biological Problems:History; Key biology research areas, Model organisms in biology (A brief overview):Genetics, Physiology, Biochemistry, Molecular Biology, Cell Biology, Genomics,Proteomics-Transcriptional regulatory network. Methods to study plant cell/tissue structure:Whole mounts, peel mounts, squash preparations, clearing, maceration and sectioning. Tissue preparation: Living versus fixed, physical versus chemical fixation, coagulating fixatives, noncoagulant fixatives; tissue dehydration using graded solvent series; Paraffin and plasticinfiltration; Preparation of thin and ultrathin sections. |
|
| 2 | Plant microtechniques:Staining procedures, classification and chemistry of stains. Staining
equipment.Reactive dyes and fluorochromes (including genetically engineered protein labeling with GFP
andother tags).Cytogenetic techniques with squashed plant materials.
The art of scientific writing and its presentation, Numbers, units, abbreviations and nomenclature used in
scientific writing. Writingreferences.Powerpoint presentations.Poster presentations. Scientific writing and ethics,Introduction to copyright-academic misconduct/plagiarism. |
|
