Syllabus
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Course Code: BOT503 Course Name: Practical BOT-501 & BOT-502 (Option- I) |
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| MODULE NO / UNIT | COURSE SYLLABUS CONTENTS OF MODULE | NOTES |
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| 1 | Study of economically important plants : Wheat, Rice, Gram, Pea, Arhar, Soybean, Black pepper, Ginger, Clove, Turmeric, Tea, Coffee, Cocoa, Cotton, Jute, Coir, Groundnut, Coconut and Mustard | |
| 2 | Collection and preparation of report on various crops and economically important plants being cultivated/wildly available in your area. | |
| 3 | Brief introduction to the components and working of the instruments- oven, autoclave, incubator, centrifuge, laminar air flow and spectrophotometer)Familiarization with basic equipments in tissue culture | |
| 4 | Preparation of MS medium. | |
| 5 | To prepare the slants and petri plates for plant tissue culture. | |
| 6 | Demonstration of in vitro sterilization and inoculation methods using leaf and nodal explants of tobacco, Datura, Brassica etc. | |
| 7 | Study of anther, embryo and endosperm culture, micropropagation, somatic embryogenesis & artificial seeds through photographs. | |
| 8 | Study of methods of gene transfer through photographs: Agrobacterium-mediated, direct gene transfer by electroporation, microinjection, microprojectile bombardment. | |
| 9 | Study of steps of genetic engineering for production of Bt cotton, Golden rice, FlavrSavr tomato through photographs. | |
| 10 | Isolation and quantification of genomic DNA from bacteria (E. coli) or Plants | |
| 11 | Study of Blotting techniques: Southern, Northern and Western, DNA fingerprinting, DNA sequencing, PCR through photographs. | |
| 12 | To separate proteins using PAGE. | |
