Syllabus
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Course Code: BOT502 Course Name: Plant Biotechnology |
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| MODULE NO / UNIT | COURSE SYLLABUS CONTENTS OF MODULE | NOTES |
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| 1 | Historical perspective of plant tissue culture; Composition of media; Nutrient and hormone requirements; Totipotency; Organogenesis; Embryogenesis; Protoplast culture; Plant tissue culture applications (micropropagation, androgenesis, virus elimination, secondary metabolite production; Cryopreservation. Restriction Endonucleases- types and role. Brief idea about cloning vectors- Ti plasmid, BAC, Lambda phage, M13phagemid, cosmid, shuttle vector; eukaryotic vectors (YAC). Recombinant DNA, bacterial transformation and selection of recombinant clones, PCR mediated gene cloning; Genomic and cDNA libraries | |
| 2 | Methods of gene transfer- Agrobacteriummediated, electroporation, microinjection,microprojectile bombardment; Selection of transgenics– selectable marker and reporter genes (Luciferase, GUS, GFP). Applications of biotechnology-pest resistant (Bt-cotton); herbicide resistant plants (Round-Upready soybean); Transgenic crops with improved quality traits (FlavrSavr tomato, golden rice); Improved horticultural varieties (Moondust carnations); Role of transgenics in bioremediation (Superbug); edible vaccines; Industrial enzymes (aspergillase, protease, lipase); Biosafety concerns. | |
