Syllabus
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Course Code: B-BTY -602 Course Name: Plant Biotechnology-II |
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| MODULE NO / UNIT | COURSE SYLLABUS CONTENTS OF MODULE | NOTES |
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| 1 | Organization of plant genome- Nuclear, Chloroplast and Mitochondrial genome.
Chloroplast Transformation- vector designing, methods and advantages. Plant Nuclear Transformation- Agrobacterium mediated transformation, Ti and Ri plasmids. Mechanism of transformation: Role of virulence genes, mechanism of T-DNA transfer, vectors based on Ti and Ri plasmids, cointegrate and binary vectors, techniques and factors effecting Agrobacterium mediated transformation of plants. Gene targeting in plants.Use of plant viruses as vectors (brief account only). Direct gene transfer methods- particle bombardment, PEG-mediated, electroporation, microinjection and Calcium phosphate mediated etc. |
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| 2 | Genetic Engineering – crop improvement, herbicide resistance, insect resistance, virus
resistance, plants as bioreactors. transgenic plants, ecological impact of transgenic
plants. Genetic modification in Food industry – background, history, controversies
over risks, application, future applications.Genetically modified foods – organic
foods, types of organic foods, identifying organic foods, organic food & preservative. Transgenic Plants: Introduction and applications. Developing insect resistance, bacterial and fungal disease resistance, virus resistance and abiotic stress tolerance in plants.Improving food quality – nutritional enhancement of plants (carbohydrates, seed storage proteins and vitamins). Production of secondary metabolites in vitro: introduction, technique and utilities. Biotransformation (a brief account only).Plant germ plasm conservation and cryopreservation. Plants as Bioreactors: antibodies, polymers, industrial enzymes. Edible vaccines |
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