Syllabus
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Course Code: BOT-303 Course Name: Plant Biotechnology & Genetic engineering |
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| MODULE NO / UNIT | COURSE SYLLABUS CONTENTS OF MODULE | NOTES |
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| 1 | Techniques used in DNA Technology: Gel Electrophoresis, PFGE, Southern and Western blotting,
Dot blots, Chemical synthesis of genes, DNA chip technology. Isolation of genes, Sequencing of genes: Maxam & Gilbert’s method, Sanger’s method and nextgeneration sequencing technologies, Brief account of proteomics and genomics |
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| 2 | DNA cloning methods, using vectors (Plasmids, phages, cosmids, phagemids, transposons, artificial
chromosomes, BAC, YAC, MAC), cloning in bacteria and eukaryotes, genomic and C-DNA
Libraries. Gene amplification by PCR: different types, DNA finger printing, molecular probes: General features and applications. |
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| 3 | Gene transfer methods in plants: plasmid mediated, electroporation, cation precipitation, liposomes,
microinjection and particles gun technology, expression of transgenes. Transgenic plants: production of transgenic plants with respect to insect resistance, herbicide resistance, resistance against biotic and abiotic factors, transgenics for male sterility and edible vaccines |
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| 4 | Yeast and algal biomass as source of single cell protein, oils and vitamins, microbial fermentation
technology in food industry. Plant and microbial biopesticides, bioremediation and phytoremediation. |
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