Syllabus
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Course Code: B-BCH –S1 Course Name: Tools and Techniques in Biochemistry |
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| MODULE NO / UNIT | COURSE SYLLABUS CONTENTS OF MODULE | NOTES |
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| 1 | Measurement of pH: Principles and composition of reference electrodes, glass electrode and
combined electrode. Hydrodynamic Methods: Sedimentation: sedimentation velocity including factors affecting it, preparative and analytical centrifugation techniques, ultracentrifugation, determination of molecular weight by hydrodynamic methods (derivations excluded and numericals included). Chromatographic techniques- General principles and applications of adsorption, ionexchange, molecular-sieve, thin layer, hydrophobic, affinity & paper chromatography. |
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| 2 | Electrophoresis- Basic principles of electrophoresis; Native & SDS-PAGE; Agarose gel
electrophoresis. Radioisotopic Techniques: Types of radiations, radioactive decay, units of radioactivity, detection and measurement of radioactivity (methods based on gas ionization and liquid scintillation counting) and Quenching. Biological hazards of radiations and safety measures in handling radioisotopes. Biological applications of radioisotopes. Spectroscopic Techniques: Beer-Lambert law, light absorption and its transmittance, extinction coefficient, a brief account of instrumentation and applications of visible and UV spectroscopic techniques (structure elucidation excluded). |
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