Syllabus
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Course Code: B-BCH-402 Course Name: Molecular Biology-II |
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| MODULE NO / UNIT | COURSE SYLLABUS CONTENTS OF MODULE | NOTES |
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| 1 | Transcription: Transcription in prokaryotes: RNA polymerase, promoters, initiation,
elongation and termination of RNA synthesis, inhibitors of transcription. Reverse transcriptase
and a brief
account of post-transcriptional processing of RNA. Translation: Genetic code- Basic features of genetic code, biological significance of degeneracy, Wobble hypothesis, split genes and overlapping genes. Mechanisms of translation: Ribosome structure; Activation of amino acids; initiation, elongation and termination of translation and Inhibitors of translation. |
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| 2 | Regulation of Gene Expression in prokaryotes: Enzyme induction and repression, Lac
operon. Recombinant DNA Technology: Introduction, steps of gene cloning, cloning vectors: features of an ideal cloning vector; plasmids, phages and cosmids as cloning vectors; ligation of insert DNA with vector; transformation of recombinant into host; selection and screening of recombinants; gene library and cDNA library. |
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