Syllabus
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Course Code: B-BCH-401 Course Name: Molecular Biology-I |
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| MODULE NO / UNIT | COURSE SYLLABUS CONTENTS OF MODULE | NOTES |
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| 1 | Basic Concepts of Genetic Information: Nucleic acids as genetic information carriers:
experimental evidences e.g. bacterial genetic transformation, Hershey-Chase experiment, TMV
reconstitution experiment. Central dogma of molecular genetics: current version. Salient
features of prokaryotic, eukaryotic and viral genomes. Histons and nucleosomes. Highly
repetitive, moderately repetitive and unique DNA sequences, telomeres, SINES, LINES, cvalue paradox, satellite DNA. DNA Supercoiling: A brief account of DNA supercoiling and topoisomerases. DNA Sequencing: Sequencing of DNA by chemical cleavage and dideoxy methods. Nucleases: Important DNases and RNases including restriction endonucleases. |
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| 2 | DNA Replication: DNA replication in prokaryotes-conservative, semiconservative and
dispersive types, experimental evidence for semiconservative replication. Enzymes and protein
factors involved in replication, mechanism of replication and inhibitors of DNA replication. Mutations and DNA Repair: Mutations: Types of mutations, Physical and chemical mutagens, Molecular basis of mutation and Ames test of carcinogenicity. DNA Repair: UV repair systems in E. coli, base-excision repair, nucleotide-excision repair & significance of thymine in DNA. |
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