Syllabus

Course Code: B-BCH-203    Course Name: Enzymes-practicals

MODULE NO / UNIT COURSE SYLLABUS CONTENTS OF MODULE NOTES
1 Estimation of protein by biuret / Lowry method
2 Assay of acid phosphatase activity from germinating mungbean seeds and calculation of specific activity of acid phosphatase.
3 Effect of enzyme concentration on enzyme activity.
4 Effect of substrate concentration on acid phosphatase activity and determination of its Km value.
5 Effect of pH on enzyme activity and determination of optimum pH.
6 Effect of Temperature on Enzyme activity.
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