Syllabus
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Course Code: B-BCH-202 Course Name: Enzymes-II |
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| MODULE NO / UNIT | COURSE SYLLABUS CONTENTS OF MODULE | NOTES |
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| 1 | Enzyme Kinetics: Factors affecting enzyme activity- enzyme concentration, substrate
concentration, pH and temperature. Derivation of Michaelis - Menten equation for uni-substrate
reactions. Km and its significance. Lineweaver-Burk plot. Importance of Kcat/Km. Bi-substrate
reactions- brief introduction of sequential and ping-pong mechanisms with examples. Reversible (competitive, non-competitive and uncompetitive inhibitions) and irreversible inhibition. Determination of Km & Vmax in the presence and absence of inhibitor. |
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| 2 | Enzyme regulation: Feedback inhibition, Allosteric enzymes. Covalently modulated enzymes. Zymogen activation. Immobilized enzymes: Advantages, methods of immobilization - Adsorption, ionic binding, covalent coupling, cross-linking, entrapment, microencapsulation etc. Applications of immobilized enzymes (A brief account). Industrial applications of enzymes (Production of glucose from starch, cellulose and dextran; use of lactase in dairy industry; production of glucose-fructose syrup from sucrose; use of protease in food, detergent and leather industry). |
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