Syllabus
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Course Code: B-BCH-201 Course Name: Enzymes-I |
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| MODULE NO / UNIT | COURSE SYLLABUS CONTENTS OF MODULE | NOTES |
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| 1 | Enzymes: Historical perspectives, general characteristics, nomenclature & classification,
significance of numbering system, holoenzyme, apoenzyme, coenzymes, cofactors, activators,
inhibitors, active site, metallo-enzymes, isoenzymes, monomeric enzymes, oligomeric enzymes,
multifunctional enzyme and multi-enzyme complexes. Enzyme specificity (absolute, group and
optical specificity), Three point attachment theory of enzyme specificity, Measurement and
expression of enzyme activity: Enzyme assay, enzyme units, enzyme turn over number and
specific activity. Role of cofactors in enzyme catalysis: NAD/NADP, FMN/FAD, coenzyme A, biocytin, Vitamin B12 Coenzyme, lipoamide, TPP, pyridoxal phosphate, tetrahydrofolate and metal ions with special emphasis on coenzyme functions. |
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| 2 | Enzyme catalysis: Reaction co-ordinate diagram, transition state, Acid-base catalysis, covalent
catalysis, proximity and orientation effects, strain and distortion theory. Mechanism of action of
chymotrypsin, carboxypeptidase,and ribonuclease. Enzyme Purification: Methods of isolation of enzymes, purification of enzymes - ammonium sulfate precipitation, molecular-sieving, ,ion-exchange chromatography, affinity chromatography, criteria of homogeneity and determination of molecular weight of enzyme. |
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