Syllabus
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Course Code: B-BCH-102 Course Name: Molecules of life-II |
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| MODULE NO / UNIT | COURSE SYLLABUS CONTENTS OF MODULE | NOTES |
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| 1 | Proteins: Introduction, classification based on solubility, shape, composition and functions. Amino acids: common structural features, stereoisomerism and RS system of designating optical isomers, classification and structures of standard amino acids as Zwitter ion in aqueous solutions, physical and chemical properties, titration of amino acids, essential amino acids and non protein amino acids. Peptides: structure of peptide bond, Merrifield solid-phase synthesis of polypeptides. Determination of the amino acid sequence of a polypeptide chain. Biological functions of polypeptides, Protein structure: levels of structure in protein architecture (Primary, secondary, tertiary and quaternary structures of proteins), Ramachandran plot and forces stabilizing these structures. Denaturation and renaturation of proteins. Salting-in and salting-out of proteins. |
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| 2 | Nucleic acids: Structures of purines pyrimidines, nucleosides and nucleotides in RNA and
DNA, generalized structural plan of nucleic acids, nomenclature used in writing structure of
nucleic acids, features of DNA double helix and forces stabilizing DNA double helix. A, B and
Z-DNAs. Chargaffs rules. Denaturation (Tm and buoyant density and their relationship with GC content in DNA) and annealing of DNA. Structure and roles of different types of RNA.
Central dogma of molecular biology. Porphyrins: Porphyrin nucleus and classification of porphyrins. Important metalloporphyrins occurring in nature. Bile pigments- chemical nature and their physiological significance. |
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