Syllabus
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Course Code: B-BTY -502 Course Name: Animal Biotechnology -II |
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| MODULE NO / UNIT | COURSE SYLLABUS CONTENTS OF MODULE | NOTES |
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| 1 | Animal Biotechnology: global perspective and new horizons Transfection:Transfection of animal cells: transfection methods. Methods for cell fusion, Selectable markers, HAT selection and Antibiotic resistance. Cloning and expression of foreign genes in animal cells: Expression vectors. Over production and preparation of the final product i.e. expressed proteins. Production of vaccines in animal cells: Hybridoma Technology: Production of monoclonal antibodies and their applications. Therapeutic products through genetic engineering – blood proteins, insulin, growth hormone etc. Gene Therapy: introduction, types of gene therapy, vectors in gene therapy, major achievements, problems and prospects. |
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| 2 | In vitro fertilization:- collection of ovaries and recovery of oocytes, selection of
oocytes for in vitro maturation , Oocyte-cumulus cell interactions, IVM, assessment
of cumulus expansion, factors affecting IVM of oocytes, semen processing, in vitro
fertilization, embryo development, Cryopreservation of oocytes and sperm; need of
cryopreservation, principle of cryopreservation, steps involved in cryopreservation,
methods, cryopreservation as strategy to establish somatic cell banking,Artificial
insemination, Animal clones.Embryo transfer technology Transgenic animals: Introduction, biopharming through animal transgenesis, Transgenic Animals: transgenic mice, sheep etc.,methods of producing transgenic animals, choices of species and tissue to produce recombinant biomolecules, limitations of large mammals transgenesis. |
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