Syllabus
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Course Code: B-BTY -501 Course Name: Animal Biotechnology-I |
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| MODULE NO / UNIT | COURSE SYLLABUS CONTENTS OF MODULE | NOTES |
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| 1 | Animal Cell & Tissue Culture: Introduction, Principles & practice. History and
Development of animal cell culture.Scope and Applications. Culture Media: Media components, Serum containing and serum free media. Natural media- Plasma clot, biological fluids, tissue extracts. Growth factors required for proliferation of animal cells. Chemically defined media, balanced salt solutions.Physical requirements for growing animal cells in culture.Washing, drying, sterilization practices, various instruments and their uses in animal cell culture practices. Cell Culture techniques: Primary Cell Culture:Initiation of cell culture-substrates (glass, plastic, metals) their preparation and sterilization. Isolation of tissue explants, disaggregation- enzyme disaggregation and mechanical disaggregation of the tissue. development of primary culture and cell lines. Subculture. Contamination.. Suspension culture, Growth curve of animal cells in culture. Secondary cell culture – transformed cell and continuous cell lines.Finite and infinite cell lines.Cell lines: Insect and animal cells. Commonly used cell lines- their organization and characteristics.Cell repositories and their function.Karyotyping, biochemical and genetic characterization of cell lines. |
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| 2 | Stem cells; main types of stem cells, embryonic stem cells, perinatal stem cells,
amniotic and placental stem cells, mesenchymal stem cells, leydig stem cells,
trophoblast stem cells, epiblast stem cells, mammary stem cells, induced pluripotent
stem cells, potential use of stem cells – cell based therapies stem cells and in vitro
derivation of gametes, stem cells in human health, porcine stem cells in human and
biomedical sciences, clinical trials update. Stem cells in livestock: brief introduction to stem cells, types of stem cells, embryonic stem cell research in livestock species, stem cells in in vitro development of gametes, status of stem cell research in livestock. Somatic cell nuclear transfer: progress in SCNT, methodology of animal cloning, , embryo development block and reprogramming in cloning, ES cells can be used for gene targeting in miceetc Cellular reprogramming and induced pluripotency: molecular basis of cellular reprogramming, gene expression in pluripotent cells, strategies for induction of genome reprogramming, reprogramming and induced pluripotency in animal sciences. |
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