Syllabus
Course Code: B-BTY -403 Course Name: Molecular Biology & Recombinant DNA Technology -Practicals |
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MODULE NO / UNIT | COURSE SYLLABUS CONTENTS OF MODULE | NOTES |
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1 | 1. Isolation of chromosomal DNA from plant cells | |
2 | 2. Isolation of chromosomal DNA from E.coli | |
3 | 3. Qualitative and quantitative analysis of DNA using spectrophotometer | |
4 | 4. Plasmid DNA isolation | |
5 | 5. Restriction digestion of DNA | |
6 | 6. Making competent cells | |
7 | 7. Transformation of competent cells. | |
8 | 8. Demonstration of PCR |