Syllabus

Course Code: B-BTY -403    Course Name: Molecular Biology & Recombinant DNA Technology -Practicals

MODULE NO / UNIT COURSE SYLLABUS CONTENTS OF MODULE NOTES
1 1. Isolation of chromosomal DNA from plant cells
2 2. Isolation of chromosomal DNA from E.coli
3 3. Qualitative and quantitative analysis of DNA using spectrophotometer
4 4. Plasmid DNA isolation
5 5. Restriction digestion of DNA
6 6. Making competent cells
7 7. Transformation of competent cells.
8 8. Demonstration of PCR
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