Syllabus
Course Code: B-BTY -401 Course Name: Molecular Biology |
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MODULE NO / UNIT | COURSE SYLLABUS CONTENTS OF MODULE | NOTES |
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1 | Basic Concepts of Genetic Information: Structure of DNA, various forces responsible
for stability of DNA, various forms of DNA, DNA topology, DNA supercoiling,
Topoisomerases in prokaryotes and eukaryotes, DNA, organization in prokaryotes
and eukaryotes, C-value paradox, denaturation: different ways for carrying out
denaturation, renaturation: requirements, various classes of DNA: highly repetitive,
moderately repetitive and unique sequence DNA replication, mutations and DNA repair: Possible modes of DNA replication, Meselson-Stahl experiment, DNA polymerases and other enzymes involved in DNA replication, Okazaki fragments, Mechanism of replication in prokaryotes and eukaryotes, inhibitors of DNA replication, molecular basis of mutations, DNA repair mechanisms like direct, base-excision, nucleotide-excision, mismatch, SOS and recombinational repair. |
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2 | Transcription and post-transcriptional modifications: RNA polymerase/s in
prokaryotes and eukaryotes, DNA footprinting technique, initiation, elongation and
termination of transcription in prokaryotes and eukaryotes, inhibitors of transcription,
RNA replicase, reverse transcriptase, post-transcriptional modifications: different
types of introns and their splicing mechanisms, processing of mRNA, rRNA and
tRNA precursors, overlapping genes and split genes. Protein synthesis, targeting and
degradation: Characteristics of the genetic code, biological significance of degeneracy, decoding the code, Wobble hypothesis, ribosomes structure and function in prokaryotes and eukaryotes, AminoacyltRNA-synthetases various factors and steps involved in protein synthesis in prokaryotes and eukaryotes, post-translational processing, signal hypothesis and protein targeting to lysosomes, Plasma membrane, extracellular matrix and different compartment of mitochondria, protein degradation. |