Syllabus
Course Code: B-BTY -303 Course Name: Practicals |
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MODULE NO / UNIT | COURSE SYLLABUS CONTENTS OF MODULE | NOTES |
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1 | solation of bacteria & their biochemical characterization | |
2 | Staining methods: simple staining, Gram staining, spore staining, negative staining, hanging drop. | |
3 | Preparation of media & sterilization methods, Methods of Isolation of bacteria from different sources. | |
4 | . Determination of bacterial cell size by micrometry | |
5 | Enumeration of microorganism - total & viable count | |
6 | Identification of pathogenic bacteria (any two) based on cultural, morphological and biochemical characteristics. | |
7 | Growth curve of a bacterium | |
8 | To perform antibacterial testing by Kirby-Bauer method. | |
9 | To prepare temporary mounts of Aspergillus and Candida by appropriate staining | |
10 | Staining methods: Gram’s staining permanent slides showing Acid fast staining, Capsule staining and spore staining. |