Syllabus
|
Course Code: BCH-307 Course Name: Practical-5 (Based on papers BCH-301 and BCH302) |
||
| MODULE NO / UNIT | COURSE SYLLABUS CONTENTS OF MODULE | NOTES |
|---|---|---|
| 1 | 1. Extraction of DNA from plant tissue/Human blood and checking of its purity | |
| 2 | 2. Preparation of plasmid DNA | |
| 3 | 3. Agarose gel electrophoresis of DNA | |
| 4 | 4. Isolation of cytoplasmic RNA | |
| 5 | 5. Electrophoresis of RNA on denaturing gels | |
| 6 | 6. Restriction digestion of DNA by restriction endonuclease | |
| 7 | 7. Construction of restriction map of plasmid DNA | |
| 8 | 8. Electrophoretic separation of isoenzymes | |
| 9 | 9. Ligation of DNA fragments | |
| 10 | 10. ELISA | |
| 11 | 11. Immunodiffusion | |
| 12 | 12. Purification of IgG from serum | |
