Syllabus
Course Code: BCH-105 Course Name: 5 Practical-1 (Based on papers BCH-101 and BCH102 |
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MODULE NO / UNIT | COURSE SYLLABUS CONTENTS OF MODULE | NOTES |
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1 | 1. To study biochemistry laboratory safety rules and guidelines | |
2 | 2. Preparation of buffers | |
3 | 3. Qualitative estimation of carbohydrates | |
4 | 4. Qualitative estimation of proteins/amino acids | |
5 | 5. Qualitative estimation of lipids | |
6 | 6. Quantitative estimation of proteins by Lowry’s method | |
7 | 7. Quantitative estimation of proteins by Bradford method | |
8 | 8. Quantitative estimation of total sugars | |
9 | 9. Quantitative estimation of reducing sugars by Nelson-Somoyogi’s method | |
10 | 10. Solubility test for lipids | |
11 | 11. To detect the presence of glycerol in given sample by acrolein method | |
12 | 12. Characterization of lipids (Acid value, Saponification value and Iodine number) | |
13 | 13. Extraction of lipids from tissues using Soxhlet’s apparatus | |
14 | 14. To determine pka of acetic acid/glycine | |
15 | 15. Subcellular fractionation of organelles from animal/plant tissue | |
16 | 16. To demonstrate: Light microscopy, Fluorescence microscopy, Confocal microscopy, Electron microscopy (scanning and transmission) |