Syllabus
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Course Code: IN-BTY -804 Course Name: Animal Cell Culture Practicals |
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| MODULE NO / UNIT | COURSE SYLLABUS CONTENTS OF MODULE | NOTES |
|---|---|---|
| 1 | 1. Preparation and sterilization of different types of cell culture media i.e. RPMI 1640, Balanced Salt solutions, MS basal media, NAM. | |
| 2 | 2. To isolate lymphocytes from whole blood by gradient centrifugation | |
| 3 | 3. To culture lymphocytes using RPMI1640 media. | |
| 4 | 4. To check cell viability by cell counting | |
| 5 | 5. To check cell viability by MTT staining | |
| 6 | 6. Demonstrate the culture of iPS | |
| 7 | 7. Demonstrate the complete process of animal cell culture including freezing, thawing, proliferations etc by taking suitable example of cell line | |
