Syllabus
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Course Code: IN-BTY -802 Course Name: Advanced Recombinant DNA Technology-Practicals |
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| MODULE NO / UNIT | COURSE SYLLABUS CONTENTS OF MODULE | NOTES |
|---|---|---|
| 1 | 1. To study in vitro DNA damage and analysis by agarose gel electrophoresis by using either purified DNA or plasmid. | |
| 2 | 2. Designing primers for PCR using online tools. | |
| 3 | 3. To study mutagenesis concept by using cancer-causing agents | |
| 4 | 4. Perform any method to be used for the selection of recombinant DNA clone | |
| 5 | 5. Gene expression in E. coli and analysis of gene product | |
| 6 | 6. Demonstration about Mammalian cell expression system with uses of CRE-LOX system | |
| 7 | 7. Demonstration about Mammalian cell expression system with uses of CRISPR/Cas9 system | |
