Syllabus
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Course Code: IN-BTY -701 Course Name: Advanced Molecular Biology |
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| MODULE NO / UNIT | COURSE SYLLABUS CONTENTS OF MODULE | NOTES |
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| 1 | Gene regulation in prokaryotes: Inducer, repressor, co-repressor and activator concept, +vely and –vely regulated genes, description of various levels of control of gene expression in prokaryotes, operon concept, lac operon: regulation by +ve and –ve mechanisms, trp operon: regulation by -ve and attenuation mechanisms, regulon, regulation of gene expression in lambda phages. Gene regulation in eukaryotes: Regulatory sequences in eukaryotes like promoter, enhancers, response elements, insulators and silencers, short-term and long term regulation of gene expression, molecular aspects of regulation of gene expression at transcription level like transcription repression by nucleosomes, histone modification by ubiquitination, acetylation, and phosphorylation, at post-transcriptional level like regulation of RNA splicing, RNA transport, RNA stability, at translational, post-translational and protein degradation level in eukaryotes. |
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| 2 | Transposable genetic elements: Discovery, mechanism of nonreplicative and replicative transposition, bacterial transposable genetic elements: simple transposons, complex transposons- the composite family and Tn3 transposon family and mechanisms of transposition, bacteriophage Mu elements. Eukaryotic transposable genetic elements – Ty elements of yeast, various autonomous and non autonomous elements of maize and mechanism of transposition. RNA world: RNA world hypothesis, messenger RNA (mRNA), transfer RNA (tRNA), ribosomal RNA (rRNA), antisense RNA, RNA as an Enzyme, as a regulator. MicroRNA (miRNA)- History of microRNA, definition, composition, Dicer, RNA induced silencing complex (RISC), modern concepts on their roles in translation inhibition. Small interfering RNA or silencing RNA (siRNA) - History of siRNA, composition and structure, roles in post-transcriptional gene silencing and potential as therapeutics Molecular Biology of Cancer: Benign and malignant tumors, types of cancers, cancer causing agents- radiations, chemical compounds, DNA and RNA viruses, mechanism of carcinogenesis, important characteristics of cancerous cells, proto-oncogenes and oncogenes, promoter insertion, enhancer insertion, chromosomal translocation, gene amplification and point mutation as mechanism for activation of proto-oncogenes. |
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