Syllabus
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Course Code: IN-BTY-601 Course Name: Genomics & Proteomics |
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| MODULE NO / UNIT | COURSE SYLLABUS CONTENTS OF MODULE | NOTES |
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| 1 | Introduction to Genomics, DNA sequencing methods – manual & automated: Maxam& Gilbert and Sangers method. Pyrosequencing, Genome Sequencing: Shotgun & Hierarchical (clone contig) methods, Computer tools for sequencing projects: Genome sequence assembly software. Managing and Distributing Genome Data: Web based servers and softwares for genome analysis: ENSEMBL, VISTA, UCSC Genome Browser, NCBI genome. Selected Model Organisms' Genomes and Databases. |
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| 2 | Introduction to protein structure, Chemical properties of proteins.Physical interactions that determine the property of proteins. Short-range interactions, electrostatic forces, van der waal interactions, hydrogen bonds, Hydrophobic interactions. Determination of sizes (Sedimentation analysis, gel filteration, SDS-PAGE); Native PAGE, Determination of covalent structures – Edman degradation. Introduction to Proteomics, Analysis of proteomes.2D-PAGE.Sample preparation, solubilization, reduction, resolution.Reproducibility of 2D-PAGE. Mass spectrometry based methods for protein identification. De novo sequencing using mass spectrometric data. |
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