Syllabus
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Course Code: IN-BTY -901 Course Name: Bioinstrumentation |
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| MODULE NO / UNIT | COURSE SYLLABUS CONTENTS OF MODULE | NOTES |
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| 1 | Spectroscopy: Raman, Fluorescence and NMR spectroscopy; ORD & CD; Mass spectrometry, MALDI-TOF, LC-MS; X-ray diffraction; Atomic absorption spectroscopy; Applications of these spectroscopic techniques in the study of Biomolecules Centrifugation: Basic principles of sedimentation; types of centrifuge (Bench top, high speed& ultracentrifuges); types of rotor; Preparative & analytical centrifugation. Separation methods-Differential centrifugation, Density gradient centrifugation; Subcellular fractionation- Disruption of cells, isolation of subcellular organelles from liver & plant cells and marker enzymes Advanced purification techniques: FPLC, HPLC |
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| 2 | Nucleic acid based techniques – Northern, Southern, Sequencing of proteins and nucleic acids, PCR, RT-PCR, QRT-PCR, DNA microarray, DNA fingerprinting (RFLP, RAPD, AFLP, SSR) Immunotechniques- Flow cytometry, Immuno-cytochemistry, immune-fluorescence and Western & Dot blots, Florescence activated cell sorter (FACS) technique, Cytotoxicity assay Biosensors - Principle and application |
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