Syllabus

Course Code: IN-BTY-504    Course Name: Plant Biotechnology- Practicals

MODULE NO / UNIT COURSE SYLLABUS CONTENTS OF MODULE NOTES
1 1. Preparation of simple growth nutrient (Knop’s medium), full strength, half strength, solid and liquid.
2 2. Preparation of complex nutrient medium (Murashige & Skoog’s medium)
3 3. To selection, Prune, sterilize and prepare an explant for culture
4 4. Haploid culture: Andogenesis and Gynogenesis.
5 5. Protoplast isolation using enzymatic method.
6 6. Analysis of various plant extracts for their antibiotic activity.
7 7. Performance of node culture.
8 8. Suspension culture with different explants.
9 9. Embryo culture.
10 10. Transferring the grown plants to hardening medium.
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