Syllabus
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Course Code: IN-BTY-503 Course Name: Plant Biotechnology |
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| MODULE NO / UNIT | COURSE SYLLABUS CONTENTS OF MODULE | NOTES |
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| 1 | Introduction: Cryo and organogenic differentiation, Types of culture: Seed , Embryo, Callus, Organs, Cell and Protoplast culture. Micropopagation Axillary bud proliferation, Meristem and shoot tip culture, cud culture, organogenesis, embryogenesis, advantages and disadvantages of micropropagation In vitro haploid production: Androgenic methods: Anther culture, Microspore culture andogenesis Sgnificance and use of haploids, Ploidy level and chromosome doubling, diplodization, Gynogenic haploids, factors effecting gynogenesis, chromosome elimination techniques for production of haploids in cereals. |
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| 2 | Protoplast Isolation and fusion: Methods of protoplast isolation, Protoplast development, Somatic hybridization, identifiation and selection of hybrid cells, Cybrids, Potential of somatic hybridization limitations.Somaclonal variation Nomenclautre, methods, applications basis and disadvantages. Organization of plant genome: Nuclear, Chloroplast and Mitochondrial genome. Chloroplast Transformation- vector designing, methods and advantages. Plant Nuclear Transformation- Agrobacterium mediated transformation, Ti and Ri plasmids, role of virulence genes, mechanism of T-DNA transfer, vectors based on Ti and Ri plasmids, cointegrate and binary vectors, techniques and factors effecting Agrobacterium mediated transformation of plants.Direct gene transfer methods- particle bombardment, PEG-mediated, electroporation, microinjection and other alternative methods. |
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