Syllabus
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Course Code: IN-BTY-502 Course Name: Recombinant DNA Technology-Practicals |
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| MODULE NO / UNIT | COURSE SYLLABUS CONTENTS OF MODULE | NOTES |
|---|---|---|
| 1 | 1. To perform plasmid isolation from E.Coli and its quality determination by agarose gel electrophoresis. | |
| 2 | 2. Designing primers in Gene Runner for PCR. | |
| 3 | 3. To perform PCR with given template and primers. | |
| 4 | 4. Demonstrate about RT-PCR | |
| 5 | 5. To perform Restriction digestion of given DNA sample. | |
| 6 | 6. Exploration of Restriction Enzyme Database REBASE | |
| 7 | 7. Drawing vector DNA map with specified features. | |
