Learning Management System

Kurukshetra University, Kurukshetra

Syllabus

Course Code: IN-BTY-S1    Course Name: Bioanalytical Tools

MODULE NO / UNIT COURSE SYLLABUS CONTENTS OF MODULE NOTES
1 Measurement of pH: Principles of glass and reference electrodes.
Hydrodynamic Methods: Chromatographic techniques- General principles and applications of adsorption, ion-exchange, molecular-sieve, thin layer, hydrophobic, affinity & paper chromatography.
2 Electrophoresis- Basic principles of electrophoresis; Native & SDS-PAGE; Agarose gel electrophoresis and Isoelectricfocussing.
Radioisotopic Techniques: Types of radiations, radioactive decay, units of radioactivity, detection and measurement of radioactivity (methods based on gas ionization and liquid scintillation counting) and Quenching. Autoradiography: overview, nuclear emulsions used in biological studies, isotopes commonly used in biochemical studies (32P, 35S, 14C and 3H), track length of emitted particles and physical arrangements between emitting source and emulsion. Biological hazards of radiations and safety measures in handling radioisotopes.Biological applications of radioisotopes.
Spectroscopic Techniques: Beer-Lambert law, light absorption and its transmittance, extinction coefficient, a brief account of instrumentation and applications of visible and UV spectroscopic techniques (structure elucidation excluded).
Copyright © 2020 Kurukshetra University, Kurukshetra. All Rights Reserved.