Syllabus

Course Code: IN-BTY-403    Course Name: Animal Biotechnology

MODULE NO / UNIT COURSE SYLLABUS CONTENTS OF MODULE NOTES
1 bAnimal Biotechnology: Scope, global perspective and new horizons, Historical perspective, and economically important livestock breeds, Model animals in animal biotechnology and genetic engineering.
Gene transfer methods in Animals: DNA transfer techniques into mammalian cells: calcium phosphate precipitation, DEAE-dextran procedure, Microinjection, Electroporation, Selectable markers, Embryonic Stem cell, gene transfer, Retrovirus & Gene transfer.
Introduction to transgenesis: Transgenic Animals – Mice, Sheep, Cow, Pig, Goat, Bird, Insect. Animal diseases need help of Biotechnology–Foot-and mouth disease, Coccidiosis, Trypanosomiasis, Theileriosis
2 Animal propagation: Artificial insemination, Animal Clones (Concepts of animal cloning, Principles and techniques of cloning).
Conservation Biology: Biotechnology in conservation of livestock diversity, Superovulation, Embryo biotechnology- Embryo collection, evaluation, and transfer, In vitro fertilization (IVF) and In vitro embryo production, Cryobanking of germplasm, oocytes and sperm, Somatic and Stem cells; Somatic nuclear transfer (SCNT), Stem technology in livestock
Genetic modification in Medicine - Gene therapy, types of gene therapy, vectors in gene therapy, molecular engineering, human genetic engineering, problems & ethics.
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