Syllabus
Course Code: IN-BTY-202 Course Name: Enzymology-Practicals |
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MODULE NO / UNIT | COURSE SYLLABUS CONTENTS OF MODULE | NOTES |
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1 | 1. Estimation of protein by Biuret / Lowry method | |
2 | 2. Assay of acid phosphatase activity from germinating mungbean seeds and calculation of specific activity of acid phosphatase. | |
3 | 3. Effect of enzyme concentration on enzyme activity. | |
4 | 4. Effect of substrate concentration on acid phosphatase activity and determination of its Km value. | |
5 | 5. Effect of pH on enzyme activity and determination of optimum pH. | |
6 | 6. Effect of Temperature on Enzyme activity. |