Syllabus

Course Code: IN-BTY-202    Course Name: Enzymology-Practicals

MODULE NO / UNIT COURSE SYLLABUS CONTENTS OF MODULE NOTES
1 1. Estimation of protein by Biuret / Lowry method
2 2. Assay of acid phosphatase activity from germinating mungbean seeds and calculation of specific activity of acid phosphatase.
3 3. Effect of enzyme concentration on enzyme activity.
4 4. Effect of substrate concentration on acid phosphatase activity and determination of its Km value.
5 5. Effect of pH on enzyme activity and determination of optimum pH.
6 6. Effect of Temperature on Enzyme activity.
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