Syllabus
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Course Code: BT-404 Course Name: Genomics, Proteomics and Metabolomics |
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| MODULE NO / UNIT | COURSE SYLLABUS CONTENTS OF MODULE | NOTES |
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| 1 | Genetic Features of Eukaryotic Nuclear Genomes -Where are the genes in a nuclear genome? How are the genes organized in a nuclear genome? How many genes are there and what are their functions? Genetic Features of Prokaryotic Genomes-How are the genes organized in a prokaryotic genome? How many genes are there and what are their functions? Prokaryotic genomes and the species concept Eukaryotic Organelle Genomes-The origins of organelle genomes, Physical features of organelle genomes, The genetic content of organelle genomes |
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| 2 | Genome Evolution-Genomes: the first ten billion years- the origins of genomes, Acquisition of new genes- by duplication events, from other species, Non coding DNA and genome evolution: Transposable elements and genome evolution, The human Genome: the last five million years Molecular Phylogenetics -origin of molecular phylogenetic, phonetics and cladistics, key features of DNA based phylogenetic trees, Applications of molecular phylogenetics- Evolutionary relationships between humans & other primates, the origins of AIDS, molecular phylogenetic as a tool in the study of human prehistory. | |
| 3 | An introduction to Proteomics, Proteome; Areas of Proteomics – Structural proteomics, Functional proteomics, Expression proteomics. Approaches for study of Proteomics: Separation of proteins by Two-dimensional electrophoresis; Mass spectrometry (ESI and MALDI); Amino acid sequencing of protein by Edman method (Traditional approach); Identification of proteins by tandem mass spectrometry; Shot gun proteomics; Protein Sequence databases; Peptide fingerprinting/mapping; Determination of 3D structure of protein by X-ray diffraction and NMR spectroscopy. Protein expression profiling – 2D differential in–gel electrophoresis, Isotope-coded affinity tag (ICAT) method for quantitative proteome analysis; Various approaches for determining the function of a protein; Protein-protein interaction using two hybrid system, complementation, tandem affinity purification (TAP) tag method; Protein-protein interaction mapping; Protein microarrays – Analytical, reverse phase, functional. |
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| 4 | Introduction to metabolism, metabolic pathways, metabolite, metabolomics; Methods/ approaches employed to study metabolism; Inter-relationship between genome, transcriptome, proteome and metabolome; Methods for measurement of metabolites level / concentration.
Metabolic regulation and control – Homeostasis and metabolic control, metabolic flux, metabolic control Analysis, Demand –Supply Analysis, mechanisms of flux control, Regulation of glycolysis in muscle as an example of metabolic regulation. Metabolic engineering – Transfer of gene/s, partial pathways, entire biosynthetic pathways for creating new products. Metabolic engineering for altering / redirecting metabolite flow. Limitations in Metabolic Engineering. |
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