Syllabus
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Course Code: BT-303 Course Name: Molecular Genetics |
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| MODULE NO / UNIT | COURSE SYLLABUS CONTENTS OF MODULE | NOTES |
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| 1 | Eukaryotic Genome Structure and Organization: Genome sequence and chromosome diversity, variation in chromosome number, Special features of metaphase chromosomes, Chromosome banding, Genome size and complexity, organization and content of human genome, Repetitive DNA, Microsatellites, genome wide repeats, Split genes, overlapping genes, cryptic genes, Retrogenes, Multigene families, Pseudo genes Nucleosome-Basic Structure, spatial arrangements of histones, chromatosome, Solenoid model, Chromatin domains, Chromatin modifications The Mutability of DNA: An overview of mutation and polymorphism, VNTR polymorphism, DNA damage- spontaneous, Induced (Alkylation, oxidation, radiation), Genotoxicity/ mutagenicity test systems - Ames test, Sister Chromatid exchanges, Micronucleus, Comet assay |
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| 2 | Transcription Regulation in Prokaryotes: Positive and Negative control of transcription, Repression and activation, Organization and regulation of Lac, Trp and Ara operon in E. coli., Organization of genome in lambda phage (early, middle and late genes), Regulation of lytic cascade, Antitermination, Repressor proteins (c1, c11, c111, cro), Establishment of lysogeny, cooperative binding of repressor, maintenance of autogenous circuit by c1 repressor Transcription Regulation in Eukaryotes: Eukaryotic activators, DNA binding domains, Transcriptional repressors, positive and negative regulation of Yeast galactose utilizing genes Signal transduction and control of transcriptional regulators, Gene silencing, Epigenetic gene regulation Regulatory RNAs: Riboswitches, Interfering RNA (RNAi) and gene expression, Short interfering RNA (siRNA) and its functions, Micro RNA and its functions, Antisense RNA and gene expression |
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| 3 | Site-Specific Recombination: Concept, Recombinases and their function, cre-lox recombination, Biological role and applications of site-specific recombination in genome manipulation Genome Mapping: DNA markers for genetic mapping, RFLP, SSP, SNPs, Physical Mapping- Restriction mapping, Florescent in situ hybridization (FISH), Sequence tagged sites (STS) mapping Genome Sequencing: High throughput sequencing, Clone by clone approach, whole genome shot gun sequencing |
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| 4 | Comparative Genomics: Concept, Orthologs and paralogs, exon shuffling, comparative genomics of eukaryotes Transcriptome Analysis: Transcriptome, SAGE, Rapid Amplification of cDNA ends (RACE), DNA microarrays Metabolic Engineering: Principle of engineering metabolic pathways, Directed production of molecules, production of novel compounds, Case studies on rerouting of metabolic pathways |
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