Syllabus
|
Course Code: BT-302 Course Name: Microbial Biotechnology |
||
| MODULE NO / UNIT | COURSE SYLLABUS CONTENTS OF MODULE | NOTES |
|---|---|---|
| 1 | Microbial Biotechnology: Scopes application and challenges. Biology of industrial micro- organisms: Industrial microorganisms, growth metabolism regulation, substrate assimilation/ product formation. Isolation and preservation of industrially important microorganisms. Fermentation system; batch and continuous system, fed batch system, multistage system. Solid state fermentation and its applications. | |
| 2 | Overproduction of primary & secondary metabolites: Use of mutation selection and recombination techniques. Fermentation raw materials: Media for industrial fermentations; criteria used in media formulation. Fermenter/bioreactor design and operation; types of fermenter, stirred tank reactor, bubble column reactor, airlift reactor, packed bed reactor, fluidized bed reactor and trickle bed reactor, agitation and aeration in a reactor, mass transfer. Foam formation and control. | |
| 3 | Industrial production of alcoholic beverages (whisky, wine and beer) and improvement by genetic engineering. Microbial production of food additives: amino acids, nucleosides and vitamins. Microbial production of industrial chemicals: Bulk organic chemicals ethanol, citric acid, acetic acid, gluconic acid, glycerol acetone and butanol. Microbial production of healthcare products: antibiotics (Penicillin & tetracyclines), Vaccines (Bacterial cells and bacterial toxins) | |
| 4 | Microbial inoculants: Food starter cultures; baker’s yeast, starter cultures for the dairy industry, meat starter cultures, Biomass production: single cell protein (SCP) production; microbial inoculants; Microbial transformation of steroids and sterols. Down-stream processing: separation processes for microbial cells and other solids, cell disruption, centrifugation, solvent recovery, drying and crystallization. Recovery schemes for non-volatile metabolites, biomass, extracellular polysaccharides and enzymes. | |
