Syllabus
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Course Code: BT-203 Course Name: Plant Cell & Tissue Culture |
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| MODULE NO / UNIT | COURSE SYLLABUS CONTENTS OF MODULE | NOTES |
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| 1 | Introduction to plant cell tissue culture and historical perspective. Laboratory organization setup (R & D level and industrial level); Aseptic manipulations and bio-safety aspects in PTC; Culture media – components, preparation and development/formulation of media for new plant system. Callus culture: characteristics, significance and limitations; Initiation and maintenance of cell cultures: static techniques of single cell culture, suspension culture and types, assessment of growth and viability of cultured cells. Organogenesis and factors influencing organogenesis. Somatic embryogenesis: process of somatic embryos production, factors influencing and its importance in plant breeding and propagation. Production of synthetic seeds. |
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| 2 | Large scale plant micropropagation – technique, factors affecting in vitro culture of plants (physical, chemical, genotypic and others), applications and limitations of micropropagation. Meristem, shoot tip culture, production and indexing of virus free plants. Somaclonal variations, molecular basis of variation and their significance in plant breeding. | |
| 3 | In vitro production of haploid plants – Androgenesis (anther and pollen culture) and Gynogenesis, Factors affecting androgenesis, ontogeny of androgenesis, diploidization of haploid plants. Significance and uses of haploids in agriculture. Wide hybridization and embryo rescue technique. | |
| 4 | Protoplast culture and somatic hybridization – Isolation, culture and fusion of protoplast, selection of fusion products, assessment of somatic hybrid plants, production of cybrids, applications of protoplast culture and somatic hybridization in the improvement of crop plants. In vitro germplasm conservation and cryopreservation. | |
