Syllabus
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Course Code: BT-202 Course Name: Animal Cell & Tissue Culture |
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| MODULE NO / UNIT | COURSE SYLLABUS CONTENTS OF MODULE | NOTES |
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| 1 | Requirements of cell & tissue culture: aseptic area, incubation, preparation and sterilization, storage, specialized equipment, consumable items. Aseptic techniques: elements of aseptic environment, sterile handling Culture vessels and substrates: the substrate, choice of culture vessel, treated surfaces. |
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| 2 | Techniques of cell culture – batch, batch fed and continuous cultures, cytotoxicity and viability assays, cell separation techniques, flow cytometry and fluorescence associated cell sorting. Design and types of media: balanced salt solutions, complete media, role of serum and supplements, serum free media: advantages and disadvantages of serum and serum free media, replacement of serum, development of serum free media. |
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| 3 | Primary culture: types of primary cell culture, isolation of the tissue, primary culture, Sub-culturing of animal cells: Subculture and propagation, Criteria for subculture, Subculture of monolayer cells, growth cycle and split ratio, propagation and subculture in suspension. Cloning and selection: dilution and suspension cloning, scaling up in suspension and monolayer, large scale production of cells using bioreactors, micro-carriers and perfusion techniques. Cell line characterization: need for characterization, authentication, cell morphology, chromosome content, DNA content, RNA and protein expression, enzyme activity, antigen markers. |
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| 4 | Industrial products of animal cell cultures: enzymes, hormones, monoclonal antibody, cytokines, tissue plasminogen activators etc. Applications of animal cell culture: virology, cancer research, gene therapy, drug development and cytotoxicity, animal cloning, genetic counselling, cryopreservation and cell banking |
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