Syllabus
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Course Code: BT-104 Course Name: Biotechniques |
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| MODULE NO / UNIT | COURSE SYLLABUS CONTENTS OF MODULE | NOTES |
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| 1 | Bioseparation, cell disruption, extraction, purification and storage techniques: Bioseparation; filtration, centrifugation, sedimentation, flocculation; Cell disruption; Liquid- liquid extraction; Purification by chromatographic techniques, reverse osmosis and ultra- filtration; Drying; Crystallization; Storage and packaging; Treatment of effluent and its disposal Centrifugation Methods: Principles of Sedimentation, centrifugation techniques and their applications, differential centrifugation, density gradient and ultracentrifugation techniques. |
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| 2 | Microscopy: Light Microscopy – Magnification, resolving power, Numerical aperture, Limit of Resolution, Principles and applications of bright field, phase contrast, fluorescence, scanning and transmission electron microscopy. Electrophoresis: Concept, Factors affecting electrophoresis, Agarose gel electrophoresis, Pulse field gel electrophoresis, PAGE, SDS-PAGE, Isoelectrofocusing, 2-Dimentional electrophoresis |
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| 3 | Chromatography: Principles and applications of Paper, Thin layer, Gel-filtration, ion- exchange, Affinity chromatography, Gas liquid chromatography, High pressure liquid chromatography (HPLC); Reversed Phase chromatography, Hydrophobic interaction chromatography. | |
| 4 | Radioisotope Techniques: Radioactivity, Units of radioactivity, Radioactive decay, Rate of radioactive decay, Measurement of radioactivity- Geiger counter, Liquid scintillation counting, Autoradiography, Effect of radiations on biological system, Cerenkov radiations, Tracer technique-Principle and applications Spectroscopy: Principles of biophysical methods used for analysis of biopolymer structure - X-ray diffraction, fluorescence, UV and visible, ORD/CD, NMR and ESR spectroscopy, Atomic absorption and Atomic emission spectroscopy. |
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