Syllabus
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Course Code: BT-103 Course Name: Molecular Cell Biology |
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| MODULE NO / UNIT | COURSE SYLLABUS CONTENTS OF MODULE | NOTES |
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| 1 | Overview of cells and cell research: Origin and evolution of cells, Cells as experimental models, tools of cell biology. Fundamentals of Molecular Biology: Heredity, Genes, and DNA, Expression of Genetic Information, Recombinant DNA, Detection of Nucleic Acids and Proteins |
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| 2 | Nucleus: Nuclear envelope and traffic between the nucleus and cytoplasm, internal organization of the nucleus, nucleolus, nucleus during mitosis. Protein Sorting and Transport: Endoplasmic reticulum, Golgi apparatus, and Lysosomes, mechanism of vesicular transport |
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| 3 | DNA Replication: DNA polymerases, replication fork, fidelity of replication, origins and initiation of replication, replication at the ends of chromosomes. Mutations: nonsense, missense, frameshift and point mutations; intragenic and intergenic suppression DNA Repair: Direct reversal of DNA damage, excision repair, error-prone repair, recombinational repair. RNA Synthesis and Processing: Prokaryotic transcription, Eukaryotic transcription: RNA polymerases and transcription factors, model systems of transcriptional control: lac operon, trp operon lambda phage; promoters, enhancers, repressors, RNA processing and turnover, Protein Synthesis, Processing and Regulation: universal genetic code, degeneracy of codons, mechanisms of initiation, elongation and termination of translation, wobble hypothesis, Protein folding and processing, regulation of protein function, protein degradation |
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| 4 | Cell Signalling: Signalling molecules and their receptors, functions of cell surface receptors, pathways of intracellular signal transduction, signal transduction and cytoskeleton, Developmental abnormalities due to defective signalling pathways, Signal transducing machinery as targets for potential drugs Cell death and cell renewal: programmed cell death, stem cells and maintenance of adult tissues. Embryonic stem cells and therapeutic cloning. Cancer: Development and causes of cancer, tumour viruses, oncogenes, tumour suppressor genes, application of molecular biology to cancer prevention and treatment. |
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