Syllabus
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Course Code: MMB-302 Course Name: Microbial Biotechnology & Industrial Microbiology |
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| MODULE NO / UNIT | COURSE SYLLABUS CONTENTS OF MODULE | NOTES |
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| 1 | Introduction to industrial microbiology: Introduction to microbial products and fermentation processes, sources of industrially important microorganisms, Industrially important microorganisms,criteria of industrially important microorganisms,Biology of industrially important microorganisms, preservation techniques for microbial cultures, inoculum development, microbial strain improvement, high throughput screening methods, recombinant DNA technology in strain improvement, oxidation-reduction principle in fermentation, recent developments in fermentation technology. | |
| 2 | Fermentation systems:
Batch cultivation, continuous cultivation, multistage chemostat, feedback systems, types of fed-batch cultures, open and closed systems, Monod kinetics of microbial growth, growth and non-growth associated product formation, product formation kinetics and mathematical modeling, bioprocess optimization strategies. Media Optimization : Substrates for industrial fermentation,media optimization strategies like Plackett–Burman design, response surface methodology. Immobilized cell reactor, solid state fermentation. |
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| 3 | Design and types of fermenters: Basic components of a fermenter, fermenter construction materials, designing of laboratory and industrial scale fermenters, types of impellers, mechanical seal, types of baffle and spargers, sampler design, foam controller, types of fermenter like stirred tank, bubble column, airlift, hollow fibers chambers, packed beds, fluidized beds, perfusion cultures, photo-bioreactors and animal cell culture bioreactor. Different types of sterilization strategies, sterilization of large scale bioreactors. Bioprocess instrumentation and control parameters: Measurement of various control parameters in bioreactor like pH, dissolved oxygen, temperature, antifoam, principles of feed-back control, PID control, respiratory quotient, effect of dissolved oxygen on microbial production processes, effect of foam and anti-foam on oxygen transfer, oxygen mass transfer coefficient, measurement of KLa values using sulfite oxidation techniques, gassing-out techniques, fluid rheology, newtonian and non-newtonian fluids, bingham plastic, pseudo plastic, power number, Reynolds number. |
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| 4 | Downstream processing: Downstream processing for filtration (DSP) cell disruption, liquid-liquid extraction, solvent recovery, supercritical fluid extraction, various chromatography techniques in product recovery, diafiltration, ultrafiltration and reverse osmosis, drying (lyophilization and spray drying), whole broth processing and crystallization, upstream processing and product recovery. Applications of bioprocessing: Production of Biofertilizers, Biopesticides, Edible Mushroom, Single Cell Protein (SCP), steroid conversion and biotransformation. Biotechnological applications of microbes in the commercial production of the following: Alcoholic beverages: Beer, Whisky Organic acids: Citric, lactic and acetic acid. Microbial enzymes: Cellulases, amylases, proteases and lipases. Antibiotics: penicillin, tetracycline Amino acids: Glutamic acid, lysine. |
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